|
| | | | |
| | | |
| Stat |
Members: 3645
Articles: 2'504'928
Articles rated: 2609
25 April 2024 |
|
| | | | |
|
Article overview
| |
|
|
Stern-Volmer Modeling of Forster Energy Transfer Between Freely
Diffusing Membrane-Bound Fluorophores | | Jeffrey T. Buboltz
; Charles Bwalya
; Dobromir Kamburov
; | | Date: |
22 May 2007 | | Subject: | Chemical Physics (physics.chem-ph); Biological Physics (physics.bio-ph) | | Abstract: | The two most common metrics used to assess Forster resonance energy transfer
(FRET) between fluorophores are (i) acceptor-quenching of donor fluorescence,
E(a.k.a. transfer efficiency); and (ii) donor-excited acceptor fluorescence,
F(A-Dex). It is still true that E is more widely used, but F(A-Dex) has been
gaining in popularity among experimentalists for practical reasons. It is
therefore notable that while a number of theoretical models have long been
available for interpreting measurements of E, the same cannot be said for
F(A-Dex). Here, for the special case of membrane-bound fluorophores, we present
a substantial body of experimental evidence that justifies the use of a simple
Stern-Volmer approach when modeling the concentration dependence of F(A-Dex)
under commonly encountered experimental conditions. Moreover, the approach
seems equally successful in modeling the acceptor-dependence of E under the
same conditions, so we have been able to make a close comparison between our
simple Stern-Volmer treatment and the first-principles analysis of 2D-FRET
published by Wolber and Hudson in 1979. Finally, Stern-Volmer modeling has
allowed us to define a linear regime of donor-acceptor dependence for F(A-Dex),
a fact which we have exploited successfully in probe-partitioning studies of
biomembrane phase behavior. | | Source: | arXiv, arxiv.0705.3218 | | Services: | Forum | Review | PDF | Favorites | |
|
|
No review found.
Did you like this article?
Note: answers to reviews or questions about the article must be posted in the forum section.
Authors are not allowed to review their own article. They can use the forum section.
browser Mozilla/5.0 AppleWebKit/537.36 (KHTML, like Gecko; compatible; ClaudeBot/1.0; +claudebot@anthropic.com)
|
| |
|
|
|
|
News, job offers and information for researchers and scientists:
| |
REGISTER