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19 April 2024 |
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Article overview
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Effects of Ox-LDL on Macrophages NAD(P)H Autofluorescence Changes by Two-photon Microscopy | | Ching-Ting Lin
; En-Kuang Tien
; Szu-Yuan Lee
; Long-Sheng Lu
; Chau-Chung Wu
; Chen-Yuan Dong
; Chii-Wann Lin
; | | Date: |
14 Aug 2007 | | Abstract: | Ox-LDL uptakes by macrophage play a critical role in the happening of
atherosclerosis. Because of its low damage on observed cells and better
signal-to- background ratio, two-photon excitation fluorescence microscopy is
used to observe NAD(P)H autofluorescence of macrophage under difference
cultured conditions- bare cover glass, coated with fibronectin or
poly-D-lysine. The results show that the optimal condition is fibronectin
coated surface, on which, macrophages profile can be clearly identified on
NAD(P)H autofluorescence images collected by two-photon microscopy. Moreover,
different morphology and intensities of autofluorescence under different
conditions were observed as well. In the future, effects of ox-LDL on
macrophages will be investigated by purposed system to research etiology of
atherosclerosis. | | Source: | arXiv, 0708.1849 | | Services: | Forum | Review | PDF | Favorites | |
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