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19 April 2024

» pubmed » pmid11237018

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A physical map of human chromosome 14
T Brüls ; G Gyapay ; J L Petit ; F Artiguenave ; V Vico ; S Qin ; A M Tin-Wollam ; C Da Silva ; D Muselet ; D Mavel ; E Pelletier ; M Levy ; A Fujiyama ; F Matsuda ; R Wilson ; L Rowen ; L Hood ; J Weissenbach ; W Saurin ; R Heilig ;
Date:	15 Feb 2001
Journal:	Nature, 409 (6822), 947-8
Abstract:	We report the construction of a tiling path of around 650 clones covering more than 99% of human chromosome 14. Clone overlap information to assemble the map was derived by comparing fully sequenced clones with a database of clone end sequences (sequence tag connector strategy). We selected homogeneously distributed seed points using an auxiliary high-resolution radiation hybrid map comprising 1,895 distinct positions. The high long-range continuity and low redundancy of the tiling path indicates that the sequence tag connector approach compares favourably with alternative mapping strategies.
Source:	PubMed, pmid11237018 doi: 10.1038/35057177
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