| | |
| | |
Stat |
Members: 3645 Articles: 2'500'096 Articles rated: 2609
18 April 2024 |
|
| | | |
|
Article overview
| |
|
A Cu(I)-semiquinone state in substrate-reduced amine oxidases | D M Dooley
; M A McGuirl
; D E Brown
; P N Turowski
; W S McIntire
; P F Knowles
; | Date: |
17 Jan 1991 | Journal: | Nature, 349 (6306), 262-4 | Abstract: | The role of copper in copper-containing amine oxidases has long been a source of debate and uncertainty. Numerous electron paramagnetic resonance (EPR) experiments, including rapid freeze-quench studies, have failed to detect changes in the copper oxidation state in the presence of substrate amines. One suggestion that copper reduction might occur, has never been confirmed. Copper amine oxidases contain another cofactor, recently identified as 6-hydroxydopa quinone (topa quinone), which is reduced by substrates. Copper has been implicated in the reoxidation of the substrate-reduced enzyme, but the failure to detect any copper redox change has led to proposals that Cu(II) acts as a Lewis acid, that it has an indirect role in catalysis, or that it serves a structural role. We present evidence for the generation of a Cu(I)-semiquinone state by substrate reduction of several amine oxidases under anaerobic conditions, and suggest that the Cu(I)-semiquinone may be the catalytic intermediate that reacts directly with oxygen. | Source: | PubMed, pmid1846226 doi: 10.1038/349262a0 | Services: | Forum | Review | Favorites |
|
|
No review found.
Did you like this article?
Note: answers to reviews or questions about the article must be posted in the forum section.
Authors are not allowed to review their own article. They can use the forum section.
browser claudebot
|
| |
|
|
|
| News, job offers and information for researchers and scientists:
| |