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28 March 2024
 
  » pubmed » pmid2812003

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Inhibition of noradrenaline release by antibodies to B-50 (GAP-43)
L V Dekker ; P N De Graan ; A B Oestreicher ; D H Versteeg ; W H Gispen ;
Date 2 Nov 1989
Journal Nature, 342 (6245), 74-6
AbstractProtein kinase C (PKC) is believed to have a crucial role in synaptic transmitter release and long-term potentiation. An important substrate of PKC in the brain is the neuron-specific presynaptically localized protein B-50 (also termed GAP-43, F1, pp46 or P-57). B-50 has been implicated in the regulation of polyphosphoinositide metabolism and calmodulin binding, and in the mechanisms of neurite outgrowth, long-term potentiation and transmitter release. It is still unknown, however, whether B-50 (and/or its phosphorylation) is essential to any of these processes. Here we report the results of studies in which antibodies to B-50, which interfere with B-50 phosphorylation, were introduced into rat cortical synaptosomes that were permeabilized with streptolysin-O (SL-O). We found that the release of [3H]noradrenaline, induced by increasing the Ca2+ concentration in the buffer, is inhibited completely by the antibodies. These results provide the first demonstration of a causal relationship between the PKC substrate B-50 and the release of neurotransmitter.
Source PubMed, pmid2812003 doi: 10.1038/342074a0
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