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Phenol stabilizes more helix in a new symmetrical zinc insulin hexamer | | U Derewenda
; Z Derewenda
; E J Dodson
; G G Dodson
; C D Reynolds
; G D Smith
; C Sparks
; D Swenson
; | | Date: |
13 Apr 1989 | | Journal: | Nature, 338 (6216), 594-6 | | Abstract: | SINCE insulin was first shown by Scott to crystallize in the presence of zinc ions in 1934, a variety of Zn-containing insulin crystals have been grown. The structures of insulin in the related rhombohedral crystals of 2Zn-insulin and 4Zn-insulin have been solved and reveal that the molecule is a hexamer, organized as three dimers, each containing a 2-fold symmetry axis and held together by Zn ions. In 2Zn-insulin the hexamer is nearly symmetrical with the two axial Zn ions and the two molecules of the dimer related closely by a local 2-fold axis. But in 4Zn-insulin the two molecules in the dimer differ remarkably, creating an asymmetric 4Zn-hexamer in which one trimer is essentially equivalent to that in 2Zn-insulin and the other is different by virtue of an additional stretch of N-terminal helix between residues B1 and B8 (refs 6, 7). We report here the structure of a new symmetrical hexamer, in which all six molecules have the B1-B8 helix seen in 4Zn-insulin. Phenol molecules, found bonding specifically to each molecule, evidently stabilize this new helical conformation. | | Source: | PubMed, pmid2648161 doi: 10.1038/338594a0 | | Services: | Forum | Review | Favorites | |
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