|
| | | | |
| | | |
| Stat |
Members: 3645
Articles: 2'501'711
Articles rated: 2609
20 April 2024 |
|
| | | | |
|
Article overview
| |
|
|
Mechanism of Na+/H+ antiporting | | Isaiah T Arkin
; Huafeng Xu
; Morten Ř Jensen
; Eyal Arbely
; Estelle R Bennett
; Kevin J Bowers
; Edmond Chow
; Ron O Dror
; Michael P Eastwood
; Ravenna Flitman-Tene
; Brent A Gregersen
; John L Klepeis
; István Kolossváry
; Yibing Shan
; David E Shaw
; | | Date: |
10 Aug 2007 | | Journal: | Science, 317 (5839), 799-803 | | Abstract: | Na+/H+ antiporters are central to cellular salt and pH homeostasis. The structure of Escherichia coli NhaA was recently determined, but its mechanisms of transport and pH regulation remain elusive. We performed molecular dynamics simulations of NhaA that, with existing experimental data, enabled us to propose an atomically detailed model of antiporter function. Three conserved aspartates are key to our proposed mechanism: Asp164 (D164) is the Na+-binding site, D163 controls the alternating accessibility of this binding site to the cytoplasm or periplasm, and D133 is crucial for pH regulation. Consistent with experimental stoichiometry, two protons are required to transport a single Na+ ion: D163 protonates to reveal the Na+-binding site to the periplasm, and subsequent protonation of D164 releases Na+. Additional mutagenesis experiments further validated the model. | | Source: | PubMed, pmid17690293 doi: 10.1126/science.1142824 | | Services: | Forum | Review | Favorites | |
|
|
No review found.
Did you like this article?
Note: answers to reviews or questions about the article must be posted in the forum section.
Authors are not allowed to review their own article. They can use the forum section.
browser Mozilla/5.0 AppleWebKit/537.36 (KHTML, like Gecko; compatible; ClaudeBot/1.0; +claudebot@anthropic.com)
|
| |
|
|
|
|
News, job offers and information for researchers and scientists:
| |
REGISTER