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Polarization of chemoattractant receptor signaling during neutrophil chemotaxis | | G Servant
; O D Weiner
; P Herzmark
; T Balla
; J W Sedat
; H R Bourne
; | | Date: |
11 Feb 2000 | | Journal: | Science, 287 (5455), 1037-40 | | Abstract: | Morphologic polarity is necessary for chemotaxis of mammalian cells. As a probe of intracellular signals responsible for this asymmetry, the pleckstrin homology domain of the AKT protein kinase (or protein kinase B), tagged with the green fluorescent protein (PHAKT-GFP), was expressed in neutrophils. Upon exposure of cells to chemoattractant, PHAKT-GFP is recruited selectively to membrane at the cell’s leading edge, indicating an internal signaling gradient that is much steeper than that of the chemoattractant. Translocation of PHAKT-GFP is inhibited by toxin-B from Clostridium difficile, indicating that it requires activity of one or more Rho guanosine triphosphatases. | | Source: | PubMed, pmid10669415 doi: 8268 | | Services: | Forum | Review | Favorites | |
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