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23 April 2024 |
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Mapping of catalytic residues in the RNA polymerase active center | | E Zaychikov
; E Martin
; L Denissova
; M Kozlov
; V Markovtsov
; M Kashlev
; H Heumann
; V Nikiforov
; A Goldfarb
; A Mustaev
; | | Date: |
5 Jul 1996 | | Journal: | Science, 273 (5271), 107-9 | | Abstract: | When the Mg2+ ion in the catalytic center of Escherichia coli RNA polymerase (RNAP) is replaced with Fe2+, hydroxyl radicals are generated. In the promoter complex, such radicals cleave template DNA near the transcription start site, whereas the beta’ subunit is cleaved at a conserved motif NADFDGD (Asn-Ala-Asp-Phe-Asp-Gly-Asp). Substitution of the three aspartate residues with alanine creates a dominant lethal mutation. The mutant RNAP is catalytically inactive but can bind promoters and form an open complex. The mutant fails to support Fe2+-induced cleavage of DNA or protein. Thus, the NAD-FDGD motif is involved in chelation of the active center Mg2+. | | Source: | PubMed, pmid8658176 | | Services: | Forum | Review | Favorites | |
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