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29 March 2024 |
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Cloning and expression of a Xenopus embryonic gap junction protein | L Ebihara
; E C Beyer
; K I Swenson
; D L Paul
; D A Goodenough
; | Date: |
3 Mar 1989 | Journal: | Science, 243 (4895), 1194-5 | Abstract: | Gap junctions in the early amphibian embryo may play a fundamental role in the regulation of differentiation by mediating the cell-to-cell transfer of chemical signals. A complementary DNA encoding a gap junction present in Xenopus oocytes and early embryos has now been cloned and sequenced. This protein sequence is homologous to the well-characterized gap junction structural proteins rat connexin32 and connexin43. RNA blot analysis of total Xenopus oocyte RNA showed hybridization to a single 1.6-kilobase band. This messenger RNA is abundant in oocytes, decreases to levels below the sensitivity of our assay by stage 15 (18 hours), and is not detectable in RNA from a number of adult organs. To confirm that the oocyte cDNA encodes a gap junction channel, the protein was over expressed in Xenopus oocytes by injection of RNA synthesized in vitro. Pairs of RNA-injected oocytes formed many more time- and voltage-sensitive cell-cell channels than water-injected pairs. | Source: | PubMed, pmid2466337 | Services: | Forum | Review | Favorites |
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