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Derivation of clones close to met by preparative field inversion gel electrophoresis | | F Michiels
; M Burmeister
; H Lehrach
; | | Date: |
5 Jun 1987 | | Journal: | Science, 236 (4806), 1305-8 | | Abstract: | The molecular analysis of genes identified by mutations is a major problem in mammalian genetics. As a step toward this goal, preparative field inversion gel electrophoresis (FIGE) was used to selectively isolate clones from the environment of genetically linked markers, and to select a subset of these clones containing sequences next to specific restriction sites rare in mammalian DNA. This approach has been used to generate a library highly enriched in sequences closely linked to the cystic fibrosis marker met. One clone derived from the end of a Not I restriction fragment containing the met sequence was analyzed in detail and localized within a long range map to a position 300 kilobase pairs 5’ of the metD sequence. | | Source: | PubMed, pmid3035716 | | Services: | Forum | Review | Favorites | |
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