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Biosynthesis of gamma globulin: studies in a cell-free system | K H Stenzel
; A L Rubin
; | Date: |
29 Jul 1966 | Journal: | Science, 153 (735), 537-9 | Abstract: | Incorporation of C(14)-amino acids into high-molecular-weight material precipitable by trichloroacetic acid indicates that microsomal cell-free systems, derived from spleens of immunized rabbits, are active in protein synthesis. Protein was made soluble by ultrasonic irradiation of the cell-free incubation mixtures, and low-molecular-weight materials were removed by dialysis and gel filtration. Chromatography and radio-immunoelectrophoresis of this soluble protein fraction reveal C(14)-labeled protein having several characteristics of gamma globulin. | Source: | PubMed, pmid4160998 | Services: | Forum | Review | Favorites |
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