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23 April 2024
 
  » pubmed » pmid4160998

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Biosynthesis of gamma globulin: studies in a cell-free system
K H Stenzel ; A L Rubin ;
Date 29 Jul 1966
Journal Science, 153 (735), 537-9
AbstractIncorporation of C(14)-amino acids into high-molecular-weight material precipitable by trichloroacetic acid indicates that microsomal cell-free systems, derived from spleens of immunized rabbits, are active in protein synthesis. Protein was made soluble by ultrasonic irradiation of the cell-free incubation mixtures, and low-molecular-weight materials were removed by dialysis and gel filtration. Chromatography and radio-immunoelectrophoresis of this soluble protein fraction reveal C(14)-labeled protein having several characteristics of gamma globulin.
Source PubMed, pmid4160998
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