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Ice Formation in Model Biological Membranes in the Presence of Cryoprotectors | M. A. Kiselev
; P. Lesieur
; A. M. Kisselev
; M. Ollivon
; | Date: |
14 Aug 2001 | Journal: | Nuclear Instruments & Methods in Physics Research A 448 (2000) 225-260 | Subject: | Biological Physics; Chemical Physics; Medical Physics; Soft Condensed Matter | physics.bio-ph cond-mat.soft physics.chem-ph physics.med-ph | Affiliation: | JINR, Russia), P. Lesieur (LURE, France), A. M. Kisselev (MIPT, Russia) and M. Ollivon (CNRS, France | Abstract: | Ice formation in model biological membranes is studied by SAXS and WAXS in the presence of cryoprotectors: dimethyl sulfoxide and glycerol. Three types of phospholipid membranes: DPPC, DMPC, DSPC are chosen for the investigation as well-studied model biological membranes. A special cryostat is used for sample cooling from 14.1C to -55.4C. The ice formation is only detected by WAXS in binary phospholipid/water and ternary phospholipid/cryoprotector/water systems in the condition of excess solvent. Ice formation in a binary phospholipid/water system creates an abrupt decrease of the membrane repeat distance by delta-d, so-called ice-induced dehydration of intermembrane space. The value of delta-d decreases as the cryoprotector concentration increases. The formation of ice does not influence the membrane structure (delta-d = 0) for cryoprotector mole fractions higher than 0.05. | Source: | arXiv, physics/0108023 | Services: | Forum | Review | PDF | Favorites |
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