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Sucrose Solutions as Prospective Medium to Study the Vesicle Structure: SAXS and SANS study | | M.A. Kiselev
; P. Lesieur
; A.M. Kisselev
; D. Lombardo
; M. Killany
; S. Lesieur
; | | Date: |
27 Oct 2001 | | Journal: | Journal of Alloys and Compounds v. 328 (2001) p. 71-76 | | Subject: | Biological Physics; Chemical Physics; Soft Condensed Matter | physics.bio-ph cond-mat.soft physics.chem-ph q-bio | | Abstract: | The possibility to use sucrose solutions as medium for X-ray and neutron small-angle scattering experiments has been explored for dimyristoylphosphatidylcholine (DMPC) vesicles and mixed DMPC/C_(12)E_(8) aggregates. The influence of sucrose concentration on phospholipid vesicles size and polydispersity has been investigated by complimentary X-ray and neutron scattering. Sucrose solutions decreased vesicle size and polydispersity and increased a contrast between phospholipid membrane and bulk solvent sufficiently for X-rays. 40% sucrose in H2O increased X-ray contrast by up to 10 times compared to pure H2O. The range of sucrose concentration 30%-40% created the best experimental conditions for the X-ray small-angle experiment with phospholipid vesicles. | | Source: | arXiv, physics/0110078 | | Services: | Forum | Review | PDF | Favorites | |
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