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Phase sensitive x-ray diffraction imaging of defects in biological macromolecular crystals | | Z W Hu
; B Lai
; Y S Chu
; Z Cai
; D C Mancini
; B R Thomas
; A A Chernov
; | | Date: |
1 Oct 2001 | | Journal: | Phys Rev Lett, 87 (14), 148101 | | Abstract: | Conventional x-ray diffraction topography is currently used to map defects in the bulk of protein crystals, but the lack of sufficient contrast is frequently a limiting factor. We experimentally demonstrate that this barrier can be circumvented using a method that combines phase sensitive and diffraction imaging principles. Details of defects revealed in tetragonal lysozyme and cubic ferritin crystals are presented and discussed. The approach enabling the detection of the phase changes of diffracted x rays should prove to be useful in the study of defect structures in a broad range of biological macromolecular crystals. | | Source: | PubMed, pmid11580675 | | Services: | Forum | Review | Favorites | |
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