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Measurements of Protein-Protein Interactions by Size Exclusion Chromatography | | J. Bloustine
; V. Berejnov
; S. Fraden
; | | Date: |
12 Jun 2003 | | Subject: | Soft Condensed Matter; Quantitative Methods | cond-mat.soft q-bio.QM | | Abstract: | A method is presented for determining second virial coefficients B_2 of protein solutions from retention time measurements in size exclusion chromatography (SEC). We determine B_2 by analyzing the concentration dependance of the chromatographic partition coefficient. We show the ability of this method to track the evolution of B_2 from positive to negative values in lysozyme and bovine serum albumin solutions. Our SEC results agree quantitatively with data obtained by light scattering. | | Source: | arXiv, cond-mat/0306346 | | Services: | Forum | Review | PDF | Favorites | |
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