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20 April 2024 |
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Article overview
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Super-resolution Imaging of Fluorescent Dipoles by Polarized Structured Illumination Microscopy | | Karl Zhanghao
; Xingye Chen
; Wenhui Liu
; Chuanyan Shan
; Kun Zhao
; Meiqi Li
; Amit Lai
; Qionghai Dai
; Peng Xi
; | | Date: |
14 Dec 2017 | | Abstract: | Fluorescent dipoles reflect the spatial orientation of the fluorophores,
which indicates structural information of the targeted proteins. As an
important super-resolution technique, Structured Illumination Microscopy (SIM)
requires three different couples of linearly polarized light to generate
high-contrast interferometric fringes, relating to polarization modulation, a
technique to measure dipole orientations. Here we reveal the inherent
connection between SIM and polarization modulation: exciting fluorescent
anisotropic specimens using a linearly polarized light is a type of structured
illumination in the dimension of dipole orientation, with polarization
modulation varying the phases of the pattern. Further, we invented polarized
SIM (pSIM) with super-resolution imaging of the fluorescent dipoles, through
expanding the spatial and polarization observable region in reciprocal space.
pSIM maintains all the advantages of SIM and can be performed directly on the
existing commercial SIM microscopes. We believe pSIM would become a powerful
and widely accessible technique in studying biological structures. | | Source: | arXiv, 1712.5092 | | Services: | Forum | Review | PDF | Favorites | |
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