|
| | | | |
| | | |
| Stat |
Members: 3645
Articles: 2'504'928
Articles rated: 2609
25 April 2024 |
|
| | | | |
|
Article overview
| |
|
|
Polymer Principles of Protein Calorimetric Two-State Cooperativity | | Huseyin Kaya
; Hue Sun Chan
; | | Date: |
29 Mar 2000 | | Journal: | Proteins: Struc. Func. & Gen., 40 (2000) 637 | | Subject: | Statistical Mechanics; Soft Condensed Matter | cond-mat.stat-mech cond-mat.soft q-bio | | Abstract: | The experimental calorimetric two-state criterion requires the van’t Hoff enthalpy $Delta H_{
m vH}$ around the folding/unfolding transition midpoint to be equal or very close to the calorimetric enthalpy $Delta H_{
m cal}$ of the entire transition. We use an analytical model with experimental parameters from chymotrypsin inhibitor 2 to elucidate the relationship among several different van’t Hoff enthalpies used in calorimetric analyses. Under reasonable assumptions, the implications of these $Delta H_{
m vH}$’s being approximately equal to $Delta H_{
m cal}$ are equivalent: Enthalpic variations among denatured conformations in real proteins are much narrower than some previous lattice-model estimates, suggesting that the energy landscape theory ``folding to glass transition temperature ratio’’ $T_{
m f}/T_{
m g}$ may exceed 6.0 for real calorimetrically two-state proteins. Several popular three-dimensional lattice protein models, with different numbers of residue types in their alphabets, are found to fall short of the high experimental standard for being calorimetrically two-state. Some models postulate a multiple-conformation native state with substantial pre-denaturational energetic fluctuations well below the unfolding transition temperature and/or predict a significant post-denaturational continuous conformational expansion of the denatured ensemble at temperatures well above the transition point. | | Source: | arXiv, cond-mat/0003479 | | Services: | Forum | Review | PDF | Favorites | |
|
|
No review found.
Did you like this article?
Note: answers to reviews or questions about the article must be posted in the forum section.
Authors are not allowed to review their own article. They can use the forum section.
browser Mozilla/5.0 AppleWebKit/537.36 (KHTML, like Gecko; compatible; ClaudeBot/1.0; +claudebot@anthropic.com)
|
| |
|
|
|
|
News, job offers and information for researchers and scientists:
| |
REGISTER