Science-advisor
REGISTER info/FAQ
Login
username
password
     
forgot password?
register here
 
Research articles
  search articles
  reviews guidelines
  reviews
  articles index
My Pages
my alerts
  my messages
  my reviews
  my favorites
 
 
Stat
Members: 3197
Articles: 2'218'097
Articles rated: 2592

27 June 2022
 
  » » arxiv » 287051

 Article forum


Genome-wide discovery of modulators of transcriptional interactions in human B lymphocytes
Kai Wang ; Ilya Nemenman ; Nilanjana Banerjee ; Andrea Califano ;
Date 15 Oct 2005
AbstractTranscriptional interactions in a cell are modulated by a variety of mechanisms that prevent their representation as pure pairwise interactions between a transcription factor and its target(s). These include, among others, transcription factor activation by phosphorylation and acetylation, formation of active complexes with one or more co-factors, and mRNA/protein degradation and stabilization processes. This paper presents a first step towards the systematic, genome-wide computational inference of genes that modulate the interactions of specific transcription factors at the post-transcriptional level. The method uses a statistical test based on changes in the mutual information between a transcription factor and each of its candidate targets, conditional on the expression of a third gene. The approach was first validated on a synthetic network model, and then tested in the context of a mammalian cellular system. By analyzing 254 microarray expression profiles of normal and tumor related human B lymphocytes, we investigated the post-transcriptional modulators of the MYC proto-oncogene, an important transcription factor involved in tumorigenesis. Our method discovered a set of 100 putative modulator genes, responsible for modulating 205 regulatory relationships between MYC and its targets. The set is significantly enriched in molecules with function consistent with their activities as modulators of cellular interactions, recapitulates established MYC regulation pathways, and provides a notable repertoire of novel regulators of MYC function. The approach has broad applicability and can be used to discover modulators of any other transcription factor, provided that adequate expression profile data are available.
Source arXiv, q-bio.MN/0510030
Services Forum | Review | PDF | Favorites   
 

No message found in this article forum.  You have a question or message about this article? Ask the community and write a message in the forum.
If you want to rate this article, please use the review section..

Subject of your forum message:
Write your forum message below (min 50, max 2000 characters)

2000 characters left.
Please, read carefully your message since you cannot modify it after submitting.

  To add a message in the forum, you need to login or register first. (free): registration page






ScienXe.org
» my Online CV
» Free


News, job offers and information for researchers and scientists:
home  |  contact  |  terms of use  |  sitemap
Copyright © 2005-2022 - Scimetrica