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E. coli recA protein-directed cleavage of phage lambda repressor requires polynucleotide | | N L Craig
; J W Roberts
; | | Date: |
3 Jan 1980 | | Journal: | Nature, 283 (5742), 26-30 | | Abstract: | The recA protein mediates both genetic recombination and several cellular responses to DNA damage, including the induction of temperate bacteriophage. Indication of phage lambda results from proteolytic cleavage of lambda repressor directed by recA protein. We show here that this cleavage reaction requires both polynucleotide and ATP. We suggest that a stoichiometric complex of recA protein and DNA is active both to destroy repressors by proteolytic cleavage and to initiate pairing of this DNA to its homologous sequence in a DNA duplex (’strand invasion’). | | Source: | PubMed, pmid6444245 | | Services: | Forum | Review | Favorites | |
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