|
| | | | |
| | | |
| Stat |
Members: 3669
Articles: 2'599'751
Articles rated: 2609
20 March 2025 |
|
| | | | |
|
Article overview
| |
|
|
Requirement of 19K form of Sonic hedgehog for induction of distinct ventral cell types in CNS explants | | E Martí
; D A Bumcrot
; R Takada
; A P McMahon
; | | Date: |
25 May 1995 | | Journal: | Nature, 375 (6529), 322-5 | | Abstract: | The identity and patterning of ventral cell types in the vertebrate central nervous system depends on cell interactions. For example, induction of a specialized population of ventral midline cells, the floor plate, appears to require contact-mediated signalling by the underlying notochord, whereas diffusible signals from the notochord and floor plate can induce ventrolaterally positioned motor neurons. Sonic hedgehog (Shh), a vertebrate hedgehog-family member, is processed to generate two peptides (M(r) 19K and 26/27K) which are secreted by both of these organizing centres. Moreover, experiments in a variety of vertebrate embryos, and in neural explants in vitro, indicate that Shh can mediate floor-plate induction. Here we have applied recombinant Shh peptides to neural explants in serum-free conditions. High concentrations of Shh bound to a matrix induce floor plate and motor neurons, and addition of Shh to the medium leads to dose-dependent induction of motor neurons. All inducing activity resides in a highly conserved amino-terminal peptide (M(r) 19K). Moreover, antibodies that specifically recognize this peptide block induction of motor neurons by the notochord. We propose that Shh acts as a morphogen to induce distinct ventral cell types in the vertebrate central nervous system. | | Source: | PubMed, pmid7753196 doi: 10.1038/375322a0 | | Services: | Forum | Review | Favorites | |
|
|
No review found.
Did you like this article?
Note: answers to reviews or questions about the article must be posted in the forum section.
Authors are not allowed to review their own article. They can use the forum section.
|
| |
|
|
|
REGISTER