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25 April 2024

» arxiv » q-bio.BM/0312035

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Ionic effect on combing of single DNA molecules and observation of their force-induced melting by fluorescence microscopy
Yu-Ying Liu ; Peng-Ye Wang ; Shuo-Xing Dou ; Wei-Chi Wang ; Ping Xie ; Hua-Wei Yin ; Xing-Dong Zhang ;
Date:	22 Dec 2003
Journal:	JOURNAL OF CHEMICAL PHYSICS, 121, 4302 (2004)
Subject:	Biomolecules \| q-bio.BM
Abstract:	Molecular combing is a powerful and simple method for aligning DNA molecules onto a surface. Using this technique combined with fluorescence microscopy, we observed that the length of lambda-DNA molecules was extended to about 1.6 times their contour length (unextended length, 16.2 micrometers) by the combing method on hydrophobic polymethylmetacrylate (PMMA) coated surfaces. The effects of sodium and magnesium ions and pH of the DNA solution were investigated. Interestingly, we observed force-induced melting of single DNA molecules.
Source:	arXiv, q-bio.BM/0312035
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