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Direct observation of steps in rotation of the bacterial flagellar motor | | Yoshiyuki Sowa
; Alexander D Rowe
; Mark C Leake
; Toshiharu Yakushi
; Michio Homma
; Akihiko Ishijima
; Richard M Berry
; | | Date: |
6 Oct 2005 | | Journal: | Nature, 437 (7060), 916-9 | | Abstract: | The bacterial flagellar motor is a rotary molecular machine that rotates the helical filaments that propel many species of swimming bacteria. The rotor is a set of rings up to 45 nm in diameter in the cytoplasmic membrane; the stator contains about ten torque-generating units anchored to the cell wall at the perimeter of the rotor. The free-energy source for the motor is an inward-directed electrochemical gradient of ions across the cytoplasmic membrane, the protonmotive force or sodium-motive force for H+-driven and Na+-driven motors, respectively. Here we demonstrate a stepping motion of a Na+-driven chimaeric flagellar motor in Escherichia coli at low sodium-motive force and with controlled expression of a small number of torque-generating units. We observe 26 steps per revolution, which is consistent with the periodicity of the ring of FliG protein, the proposed site of torque generation on the rotor. Backwards steps despite the absence of the flagellar switching protein CheY indicate a small change in free energy per step, similar to that of a single ion transit. | | Source: | PubMed, pmid16208378 doi: 10.1038/nature04003 | | Services: | Forum | Review | Favorites | |
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