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26 April 2024 |
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Role for c-Abl tyrosine kinase in growth arrest response to DNA damage | | Z M Yuan
; Y Huang
; Y Whang
; C Sawyers
; R Weichselbaum
; S Kharbanda
; D Kufe
; | | Date: |
18 Jul 1996 | | Journal: | Nature, 382 (6588), 272-4 | | Abstract: | The c-Abl protein tyrosine kinase is activated by certain DNA-damaging agents, and its overexpression causes arrest in the G1 phase of the cell cycle by a mechanism dependent on the tumour-suppressor protein p53 (refs 2-4). Here we investigate the possible role of c-Abl in growth arrest induced by DNA damage. Transient transfection experiments using wild-type or inactivated c-Abl show that both induce expression of p21, an effector of p53, but only wild-type c-Abl downregulates the activity of the cyclin-dependent kinase Cdk2 and causes growth arrest. Exposure to ionizing radiation of cells that stably express active or inactive c-Abl is associated with induction of c-Abl/p53 complexes and p21 expression. However, cells expressing the dominant-negative c-Abl mutant and cells lacking the c-abl gene are impaired in their ability to downregulate Cdk2 or undergo G1 arrest in response to ionizing radiation. We also show that expression of c-Abl kinase in p21(-1-), but not in p53(-1-), cells results in downregulation of Cdk2. Our results suggest that c-Abl kinase contributes to the regulation of growth arrest induced by ionizing radiation by a p53-dependent, p21-independent mechanism. | | Source: | PubMed, pmid8717045 doi: 10.1038/382272a0 | | Services: | Forum | Review | Favorites | |
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