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Heterochromatic silencing and HP1 localization in Drosophila are dependent on the RNAi machinery | | Manika Pal-Bhadra
; Boris A Leibovitch
; Sumit G Gandhi
; Madhusudana Rao
; Utpal Bhadra
; James A Birchler
; Sarah C R Elgin
; | | Date: |
30 Jan 2004 | | Journal: | Science, 303 (5658), 669-72 | | Abstract: | Genes normally resident in euchromatic domains are silenced when packaged into heterochromatin, as exemplified in Drosophila melanogaster by position effect variegation (PEV). Loss-of-function mutations resulting in suppression of PEV have identified critical components of heterochromatin, including proteins HP1, HP2, and histone H3 lysine 9 methyltransferase. Here, we demonstrate that this silencing is dependent on the RNA interference machinery, using tandem mini-white arrays and white transgenes in heterochromatin to show loss of silencing as a result of mutations in piwi, aubergine, or spindle-E (homeless), which encode RNAi components. These mutations result in reduction of H3 Lys9 methylation and delocalization of HP1 and HP2, most dramatically in spindle-E mutants. | | Source: | PubMed, pmid14752161 doi: 10.1126/science.1092653 | | Services: | Forum | Review | Favorites | |
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