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26 April 2024

» pubmed » pmid17754551

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Rapid Flow Cytometric Analysis of the Cell Cycle in Intact Plant Tissues
David W Galbraith ; Kristi R Harkins ; Joyce M Maddox ; Nicola M Ayres ; Dharam P Sharma ; Ebrahim Firoozabady ;
Date:	3 Jun 1983
Journal:	Science, 220 (4601), 1049-1051
Abstract:	Mechanical chopping of plant tissues in the presence of mithramycin released intact nuclei representative of the cells within the tissues. The amount of nuclear DNA in the homogenates of monocotyledonous and dicotyledonous plants was accurately and rapidly determined by flow microfluorometry, and the distribution of nuclei involved in the cell cycle was charted for tissues selected from different physical locations or developmental stages.
Source:	PubMed, pmid17754551 doi: 10.1126/science.220.4601.1049
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