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Short-range spectroscopic ruler based on a single-molecule optical switch | Mark Bates
; Timothy R. Blosser
; Xiaowei Zhuang
; | Date: |
14 Feb 2005 | Subject: | Quantitative Methods; Biomolecules; Biological Physics; Chemical Physics | q-bio.QM physics.bio-ph physics.chem-ph q-bio.BM | Abstract: | We demonstrate a novel all-optical switch consisting of two molecules: a primary fluorophore that can be switched between a fluorescent and a dark state by light of different wavelengths, and a secondary chromophore that facilitates switching. The interaction between the two molecules exhibits a distance dependence much steeper than that of Forster resonance energy transfer. This enables the switch to act as a ruler with the capability to probe distances difficult to access by other spectroscopic methods, thus presenting a new tool for the study of biomolecules at the single-molecule level. | Source: | arXiv, q-bio.QM/0502012 | Other source: | [GID 234355] pmid15783528 | Services: | Forum | Review | PDF | Favorites |
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