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26 April 2024
 
  » arxiv » q-bio.QM/0502012

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Short-range spectroscopic ruler based on a single-molecule optical switch
Mark Bates ; Timothy R. Blosser ; Xiaowei Zhuang ;
Date 14 Feb 2005
Subject Quantitative Methods; Biomolecules; Biological Physics; Chemical Physics | q-bio.QM physics.bio-ph physics.chem-ph q-bio.BM
AbstractWe demonstrate a novel all-optical switch consisting of two molecules: a primary fluorophore that can be switched between a fluorescent and a dark state by light of different wavelengths, and a secondary chromophore that facilitates switching. The interaction between the two molecules exhibits a distance dependence much steeper than that of Forster resonance energy transfer. This enables the switch to act as a ruler with the capability to probe distances difficult to access by other spectroscopic methods, thus presenting a new tool for the study of biomolecules at the single-molecule level.
Source arXiv, q-bio.QM/0502012
Other source [GID 234355] pmid15783528
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