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Serological detection of mixed lymphocyte culture identity between cells that differ by one HL-A haplotype | | T Kovithavongs
; L Hyshka
; P R McConnachie
; J B Dossetor
; | | Date: |
20 Dec 1974 | | Journal: | Science, 186 (4169), 1124-6 | | Abstract: | Antibody mediated cell dependent immune lympholysis (ABCIL), an extremely sensitive serological technique for detecting tissue sensitization, was used in a family segregation study. Two serums capable of demonstrating ABCIL were used to identify members of the family who differed by one HL-A haplotype but whose cells did not stimulate in mixed lymphocyte culture. Absorption study of one serum indicated that the ABCIL reaction of that serum was directed against a factor, independent of HL-A, that might be responsible for lymphocyte stimulation in mixed lymphocyte culture. Thus the ABCIL technique may be used to detect lymphocyte-defined gene products. | | Source: | PubMed, pmid4283385 | | Services: | Forum | Review | Favorites | |
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